Gli1-CreERT2

모델명

C57BL/6Smoc-Gli1em1(CreERT2-Wpre-polyA)Smoc

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NM-KI-200073

모델 상태

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유전자 요약

Official Symbol
Gli1

Synonyms

Zfp5; Zfp-5; AV235269

NCBI ID

14632

MGI ID

95727

Ensembl ID

ENSMUSG00000025407

Pubmed

Gli1

Human Ortholog

GLI1

모델 설명

A CreERT2-Wpre-polyA expression cassette was knocked into the Gli1 gene start codon site. Gli1 encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. When these Gli1-CreERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Gli1 expressing cells; making them useful for studying axis patterning, proliferation, and cell fate specification of Hedgehog responding cells at different stages of embryogenesis.
신청분야：Cre recombinase tool; Hedgehog signal pathway

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Fig1 CreERT2-mediated recombination in Gli1-CreERT2; Rosa26-tdTomato mice after tamoxifen treatment. At one month of age, tdtomato are detectable within the suture mesenchyme of craniofacial bones. (A) Whole-mount labeling of tdTomato of calvarial bones of one-month-old Gli1-CreERT2; Rosa26-tdTomato mice. （B）Labeling of sections of sagittal sutures and parietal bones of one-month-old mice.

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Fig. 2 Labeling was also observed in cortex, hippocampus，thalamus, some cells of pituitary gland, submandibular gland, kidney, ovary, large intestine, small intestine, skin follicles, alveoli, bronchi, individual cells of liver, heart, muscle, and spleen, but not in pancreas and thymus.（For more information please contact: 400-728-0660.）


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Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

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Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.

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