Bhlha15-CreERT2

모델명

C57BL/6Smoc-Bhlha15em1(CreERT2)Smoc

카탈로그

NM-KI-200080

모델 상태

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유전자 요약

Official Symbol
Bhlha15

Synonyms

Mist1; Bhlhb8; MIST-1; 1810009C13Rik

NCBI ID

17341

MGI ID

891976

Ensembl ID

ENSMUSG00000052271

Pubmed

Bhlha15

Human Ortholog

BHLHA15

모델 설명

The coding region of Bhlha15 gene was replaced by a CreERT2 expression cassette.Besides,a kozak-CreERT2-WPRE-polyA expression cassette were knock into the Bhlha15 gene start codon site to generate Bhlha15-CreERT2(2)(Stock No. NM-KI-200158) mice. Bhlha15(basic helix-loop-helix family member a15) is also known as Mist1. The gene product is involved in controlling exocrine secretion from the pancreas, small intestine and stomach. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cells expressing Bhlha15.
신청분야：Cre recombinase tool, pancreatic acinar cells

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Fig. 1 CreERT2-mediated recombination in the intestine of Bhlha15CreERT2/+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the crypt of Bhlha15CreERT2/+; Rosa26tdTomato/+ mouse after tamoxifen treatment. 

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Fig. 2 CreERT2-mediated recombination in the pancreas of Bhlha15CreERT2/+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the pancreas of Bhlha15CreERT2/+; Rosa26tdTomato/+ mouse after tamoxifen treatment. Some leakiness can be detected in the pancreas prior to tamoxifen treatment.

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Fig. 3 Detection of tdTomato(red) in various tissues of Bhlha15CreERT2/+; Rosa26tdTomato/+ mice. Tdtomato is expressed in the pancreas, pituitary, stomach, crypt, salivary gland, lung, spleen, liver and kidney after tamoxifen treatment. Some leakiness can be detected in the salivary gland and pancreas prior to tamoxifen treatment. Tdtomato expression can not be observed in the brain, colon, heart, retina and skin. (For more detailed information please contact our technical advisor.)

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Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

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Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.

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