Nos1-CreERT2

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C57BL/6Smoc-Nos1em1(CreERT2-SV40polyA)Smoc

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NM-KI-200091

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Official Symbol
Nos1

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A CreERT2-SV40polyA expression cassette was knocked into the Nos1 gene start codon site.
신청분야:Nos1, the neurogenic nitric oxide synthase, is a key enzyme responsible for the production of the signaling molecule nitric oxide (NO) by neurons. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cells expressing cre after tamoxifen treatment.

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NM-KI-200091;Nos1-CreERT2-1.png

Fig. 1 CreERT2-mediated recombination in the brian of Nos1CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the NOS1 positive neurons of Nos1CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment. 

NM-KI-200091;Nos1-CreERT2-2.png

Fig. 2 CreERT2-mediated recombination in the large intestine of Nos1CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the muscular layer of the wall of the large intestine derived from Nos1CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment. 

NM-KI-200091;Nos1-CreERT2-3.png

Fig. 3 CreERT2-mediated recombination in the small intestine of Nos1CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the muscular layer of the wall of the small intestine derived from Nos1CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment.

NM-KI-200091;Nos1-CreERT2-4.png

Fig. 4 Detection of tdTomato(red) in various tissues of Nos1CreERT2/+; Rosa26tdTomato/+ mice. CreERT2 mediated recombination can be detected in some cells of the brain and intestine. Some leakiness were detected prior to tamoxifen exposure. Besides, tamoxifen-dependent cre recombinase expression can also be detected in some cells derived from eyes, lung, submandibular gland, kidney, spleen, thymus, muscle and ovary, except for heart, brown fat tissue and liver. (For more detailed information please contact our technical advisor.)


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Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

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