Cd4-IRES-DTRGFP

모델명

C57BL/6Smoc-Cd4em5(IRES-DTREGFP)Smoc

카탈로그

NM-KI-190121

모델 상태

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유전자 요약

Official Symbol
Cd4

모델 설명

A IRES-DTREGFP expression cassette was knocked into the Cd4 gene stop codon site.
신청분야:DTR mouse

검증 데이터

CD4-DTR.png

Figure 1.  EGFP expression analysis in  PBMC of CD4-IRES-DTR-EGFP heterozygous mice by FACS. (In collaboration with CrownBio).

验证数据-CD4-IRES-DTR-EGFP-图片2.png

Figure2. The percent of  CD4+ T cells and other immune cells in peripheral blood of CD4-IRES-DTR-EGFP mice were detected by FACS. (In collaboration with CrownBio) 

The C57BL/6 mice and CD4-IRES-DTR-EGFP mice were injected with diphtheria toxin, the results indicated that CD4+ T cells and Tregs of CD4-IRES-DTR-EGFP mice were depleted and meanwhile the amount of CD8+ T cells increased.

验证数据-CD4-IRES-DTR-EGFP-图片3.png

Figure3. The growth of MC38 tumors in CD4-IRES-DTR-EGFP mice and C57BL/6 mice. (In collaboration with CrownBio)

Heterozygous CD4-IRES-DTR-EGFP mice and C57BL/6 mice were inoculated with MC38 colon cancer cells.  The CD4-IRES-DTR-EGFP mice were injected with diphtheria toxin once in two days.


당신은 또한 좋아할 수 있습니다

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

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Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.

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