hNOTCH3
모델명
C57BL/6JSmo-Notch3tm(hNOTCH3)Smoc
카탈로그
NM-HU-230037
모델 상태
Repository Live
유전자 요약
Gene Symbol
Notch3
모델 설명
검증 데이터
Fig.1 Detection of NOTCH3 expression in lung by RT-PCR.
Lung RNA was extracted from wild-type C57BL/6 mice (WT) and homozygous hNOTCH3 knockin mice (HO), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human Notch3 primers (n=1-3, female, 10 weeks old). Mouse Notch3 mRNA (255 bp) was detectable only in wild-type C57BL/6 mice. Human NOTCH3 mRNA (141 bp) was detectable only in homozygous hNOTCH3 knockin mice but not in wild-type mice.
Fig.2 Detection of NOTCH3 expression in hNOTCH3 mice by WB.
The lung and mammary gland tissue lysates were collected from 10-week-old female wild-type C57BL/6 mice (WT) and 10-week-old female homozygous hNOTCH3 mice (HO), and then analyzed by western blot with anti-NOTCH3 antibody (CST, 5276T).
The antibody is cross-reactive between human and mouse NOTCH3. PC, positive control, MCF7 cells.
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Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
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At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.
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