HLA-DPA1/HLA-DPB1
모델명
C57BL/6JSmo-H2-Aatm1(HLA-DPA1*02:02)H2-Ab1tm1(HLA-DPB1*03:01)Smoc
카탈로그
NM-HU-234679
모델 상태
Repository Live
유전자 요약
Gene Symbol
H2-Aa
모델 설명
검증 데이터
Fig.1 Detection of HLA-DPA1/HLA-DPB1 expression in large intestine and spleen by RT-PCR.
Wild type: one band at 278 bp with primers F4/R4(mH2-Aa) and one band at 123 bp with primers F/R(mGapdh);
Heterozygous: one band at 278 bp with primers F4/R4(mH2-Aa) and one band at 107 bp with primers F1/R1(hHLA-DPA1) and one band at 123 bp with primers F/R(mGapdh).
Abbr. M, DNA marker; HE, heterozygous; WT, wild type.
Fig.2 Detection of HLA-DPA1/HLA-DPB1 expression in large intestine and spleen by RT-PCR.
Wild type: one band at 299 bp with primers F3/R3(mH2-Ab1) and one band at 123 bp with primers F/R(mGapdh);
Heterozygous: one band at 299 bp with primers F3/R3(mH2-Ab1), one band at 113 bp with primers F2/R2(hHLA-DPB1) and one band at 123 bp with primers F/R(mGapdh).
Abbr. M, DNA marker; HE, heterozygous; WT, wild type.
Fig.3 Detection of MHC-Ⅱ expression on B cells and DCs in Peripheral blood in 13-week-old WT C57BL/6 and HE HLA-DPA1/HLA-DPB1 mice.
Fig.4 Detection of MHC-Ⅱ expression on B cells and DCs in Spleen in 13-week-old WT C57BL/6 and HE HLA-DPA1/HLA-DPB1 mice.
Fig.5 Detection of MHC-II expression on B cells in blood of WT C57BL/6 mice and HO HLA-DPA1/HLA-DPB1 mice by FACS. Leukocytes were collected from wild-type C57BL/6 mice (male, 6 weeks old) and homozygous HLA-DPA1/HLA-DPB1 humanized mice (male, 6 weeks old), and analyzed by flow cytometry with anti-mMHC-II and anti-hMHC-II. hMHC-II was exclusively detectable in HLA-DPA1/HLA-DPB1 humanized mice but not in wild-type mice. hMHC-II was exclusively detectable on B cells in HLA-DPA1/HLA-DPB1 humanized mice, whereas mMHC-II was exclusively detectable on B cells in wild-type C57BL/6 mice.
Abbr. HO, homozygous; WT, wild type.
Fig.6 Detection of MHC-II expression on B cells and DCs in spleen of WT C57BL/6 mice and HO HLA-DPA1/HLA-DPB1 mice by FACS. Leukocytes were collected from wild-type C57BL/6 mice (male, 6 weeks old) and homozygous HLA-DPA1/HLA-DPB1 humanized mice (male, 6 weeks old), and analyzed by flow cytometry with anti-mMHC-II and anti-hMHC-II. hMHC-II was exclusively detectable in HLA-DPA1/HLA-DPB1 humanized mice but not in wild-type mice. hMHC-II was exclusively detectable on B cells and DCs in HLA-DPA1/HLA-DPB1 humanized mice, whereas mMHC-II was exclusively detectable on B cells and DCs in wild-type C57BL/6 mice.
Abbr. HO, homozygous; WT, wild type.
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Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
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