Ptf1a-CreERT2

모델명

C57BL/6Smoc-Ptf1aem1(CreERT2-WPRE-pA)Smoc

카탈로그

NM-KI-200118

모델 상태

Repository Live

PDF 받기

유전자 요약

Official Symbol
Ptf1a

Synonyms

PTF1p48; bHLHa29; PTF1-p48

NCBI ID

19213

MGI ID

1328312

Ensembl ID

ENSMUSG00000026735

Pubmed

Ptf1a

Human Ortholog

PTF1A

모델 설명

A CreERT2-WPRE-pA expression cassette was knocked into the Ptf1a gene start codon site.
신청분야：These mice express cre recombinase from the Ptf1a locus. By mating the reporter mice with CreERT2-expressing mice, reporter gene expression can be detected in the pancreatic acinar cells of the Ptf1aCreERT2/+ ; Rosa26tdTomato/+ mouse. And the mice are useful for generating conditional mutations in pancreatic acinar cells. This strain may be useful for in the research of pancreatic cancer.

검증 데이터

Ptf1a-CreERT2-1.png

Fig.1 CreERT2-mediated recombination in the pancreas of Ptf1aCreERT2/+ ; Rosa26tdTomato/+ mouse. 

TdTomato(red) expression can be detected in the pancreatic acinar cells of Ptf1aCreERT2/+; Rosa26tdTomato/+ mouse after tamoxifen treatment. 

Ptf1a-CreERT2-2.png

Fig.2 Detection of tdTomato (red) in various tissues of Ptf1aCreERT2/+; Rosa26tdTomato/+ mice after tamoxifen treatment. 

CreERT2-mediated recombination in the pancreatic acinar cells  can be induced by tamoxifen. Some leakiness were detected in the pancreas prior to tamoxifen exposure. Besides, tdtomato expression can be detected in a few cells of the brain, pituitary gland and stomach. Tdtomato exprssion can not be detected in thymus, heart, lung, liver, testis, kidney, intestine, brown fat, spleen, submandibular gland, and muscle. (For more detailed information please contact our technical advisor.)

당신은 또한 좋아할 수 있습니다

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

조회
Milestones

Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.

조회