hCB1(2)
모델명
C57BL/6Smoc-Cnr1em1(hCNR1)Smoc
카탈로그
NM-HU-225070
모델 상태
Embryo cryopreservation
유전자 요약
Official Symbol
Cnr1
모델 설명
The endogenous mouse Cnr1 gene was replaced by human CNR1 gene.
검증 데이터
Fig1. Detection of CB1 expression in cerebellum by RT-PCR.
Wild type: only one band at 373 bp with primers F1/R1(mCb1);
Homozygous: only one band at 209 bp with primers F2/R2(hCB1).
Abbr. M, DNA marker; HO, homozygous; WT, wild type.
Fig2. Detection of human CB1 expression in Brains, liver and kidney in hCB1 knockin mice by WB.
Abbr. HO, homozygous; WT, wild type.
Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.
Fig3. Detection of human CB1 expression in CA1 in hCB1(2) knockin mice by IF.
Brains were collected from wild-type mice and HO hCB1(2) mice, and analyzed by IF with anti-human CB1 antibody.
Abbr. HO, homozygous; WT, wild type.
Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.
Fig4. Detection of human CB1 expression in CA3 in hCB1(2) knockin mice by IF.
Brains were collected from wild-type mice and HO hCB1(2) mice, and analyzed by IF with anti-human CB1 antibody.
Abbr. HO, homozygous; WT, wild type.
Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.
Fig5. Detection of human CB1 expression in Dentate Gyrus(DG) in hCB1(2) knockin mice by IF.
Brains were collected from wild-type mice and HO hCB1(2) mice, and analyzed by IF with anti-human CB1 antibody.
Abbr. HO, homozygous; WT, wild type.
Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.
Fig6. Detection of human CB1 expression in Cerebellum in hCB1(2) knockin mice by IF.
Brains were collected from wild-type mice and HO hCB1(2) mice, and analyzed by IF with anti-human CB1 antibody.
Abbr. HO, homozygous; WT, wild type.
Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.
당신은 또한 좋아할 수 있습니다
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
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