hC3

The endogenous mouse C3 gene was replaced by human C3 gene.

Fig1. Detection of C3 expression in liver by RT-PCR. 

Wild type: only one band at 359 bp with primers F1/R1(mC3);

Heterozygous: one band at 359 bp with primers F1/R1(mC3) and one band at 357 bp with primers F2/R2(hC3); 

Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

Fig 2. Detection of heterozygous hC3 expression in serum by ELISA. 

Abbr. Het, heterozygous; WT, wild type.

Note: Data from the collaborator.

Fig 3. Detection of hC3 expression in serum by ELISA(n=3).

Abbr. HO, homozygous; HE, heterozygous; WT, wild type.

Fig 4. Detection of hC3 expression in eyes in hom hC3 KI mice by IF (n=5/group).

Fig 5. The C3hu/hu mice showed inflammation in the portal region and bile duct hyperplasia (100× and 400×)

Fig 6. The C3hu/hu mice showed glomerular sclerosis, glomerular atrophy and inflammation of the capillary tufts area (100× and 400×)

Fig 7. Body Weight of C3hu/hu mouse (n=12/group).

Fig 8. Survival curve of C3hu/hu mouse (n=12/group).

Fig 9. Serum Biochemistry of C3hu/hu mouse (n=5/group).

Fig 10. Serum Biochemistry of C3hu/hu mouse (n=5/group, a different biochemical analyzer was used than in Fig 9).

Fig 11. Urinalysis of C3hu/hu mouse.