R26-CAG-LSL-Luc-tdTomato

모델명

C57BL/6Smoc-Gt(ROSA)26Sorem1(CAG-LSL-luci-2A-tdTomato)Smoc

카탈로그

NM-KI-18051

모델 상태

Repository Live

PDF 받기

유전자 요약

Official Symbol
Gt(ROSA)26Sor

Synonyms

R26，ROSA26，AV258896，Gtrgeo26，Gtrosa26，Thumpd3as1

NCBI ID

14910

MGI ID

104735

Ensembl ID

ENSMUSG00000086429

Pubmed

Gt(ROSA)26Sor

모델 설명

A CAG-loxP STOP-loxP-luci-2A-tdTomato-WPRE-polyA cassette was knocked into the mouse ROSA26 locus . This strain of mice can be mated with mice expressing Cre recombinase so that luciferase and tdTomato fluorescent protein could be expressed for cell tracing.
신청분야：Cell tracing

검증 데이터

图1.png

Fig1. In the absence of any stimulation, the in vivo imaging system detected that R26-CAG-LSL-luci-tdTomato(+/-)；Dppa3-Cre (+/-) mice showed significant fluorescence.

图2.png

Fig2. The in vivo imaging system detected that R26-CAG-LSL-luci-tdTomato(+/-) mice showed some fluorescence. Some 'leaking' was found where luciferase were being expressed at a low level even without Cre.

image.png

Fig. 3 Detection of tdTomato(red) in the liver, stomach, small intestine and large intestine of Dppa3Cre/+; Rosa26Luc-tdTomato/+ mice. 

image.png

Fig. 4 Detection of tdTomato(red) in various tissues of Dppa3Cre/+; Rosa26Luc-tdTomato/+ mice. Tdtomato was expressed in the brain, eye, heart, spleen, kidney, pancreas, thymus, salivary glands, lung, skin, spinal cord, testis and epididymis. (For more detailed information please contact our technical advisor.)

당신은 또한 좋아할 수 있습니다

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

조회
Milestones

Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.

조회