Rag1-KO(Rag1-EGFP)

모델명

B6;129S-Rag1tm1(loxP-EGFP-PolyA-loxP-Neo-loxP)Smoc

카탈로그

NM-KI-00069

모델 상태

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유전자 요약

Official Symbol
Rag1

Synonyms

Rag-1

NCBI ID

19373

MGI ID

97848

Ensembl ID

ENSMUSG00000061311

Pubmed

Rag1

Human Ortholog

RAG1

Human Ortholog Associated Diseases by GWAS 

奥门综合症、免疫缺陷综合症

모델 설명

A loxP-EGFP-PolyA-loxP-Neo-loxP expression cassette was knocked into the Rag1 gene start codon site. As a Rag1 knockout mouse model, this stain can be used in subcutaneous inoculation of liver cancer tissues and tumor cells. Tumors can esaily form and grow. The amount of T and B lymphocytes in peripheral blood of mice was extremely low tested by FACS, which was comparable to or lower than that of Nude mice, and there was a significant difference compared with wild type mice. The pathological sections of HE staining of tumor tissues showed that the tumor sections of Rag1 KO mice and Nude mice were similar. This strain has the potential to replace Nude, NOD-SCID mice as a tumor-bearing mouse model.
신청분야：Immunodeficiency,tumor-bearing model

검증 데이터

rag1.jpg

Figure 1. Generation strategy of Rag1 gene knockout mice.


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Figure 2  Splenocytes cells of C57BL/6J, NOD-SCID, Rag1-/-, and Rag2-/-  mice were isolated. Fractions of T and B cells were characterized using flow cytometry. 


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Figure3. Complete deletion of T and B cells in the blood of Rag1-KO/ Rag2-KO mice.

(A) The peripheral blood samples of C57BL/6, Rag1-KO and Rag2-KO mice were collected to analyze their compositions of T, B and NK cells by FACS.(B) Statistical analysis of sorted cells.

rag1-rag2-脾脏.png

Figure4. Complete deletion of T and B cells in the spleen of Rag1-KO/ Rag2-KO mice.

(A) The splenocytes of C57BL/6, Rag1-KO and Rag2-KO mice were collected to analyze their compositions of T, B and NK cells by FACS.(B) Statistical analysis of sorted cells.

rag1-rag2-胸腺.png

Figure5. Complete deletion of T and B cells in the thymus gland of Rag1-KO/ Rag2-KO mice.

(A) The thymocyte of C57BL/6, Rag1-KO and Rag2-KO mice were collected to analyze their compositions of T, B and NK cells by FACS.(B) Statistical analysis of sorted cells.

Rag1-Rag2.png

Figure6.The establishment of tumor models using A549 lung cancer cells is more effective in Rag1-/- or Rag2-/-   mice.

Table 1. Blood routine tests in Rag1-KO(Rag1-EGFP).

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Table2. Blood biochemistry in Rag1-KO(Rag1-EGFP).

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간행물

Programmed death protein 1 is essential for maintaining the anti-inflammatory function of infiltrating regulatory T cells in a murine spinal cord injury model
References：Journal of Neuroimmunology

Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus
References：Nature Communications

Interleukin-17 Regulates Neuron-Glial Communications, Synaptic Transmission, and Neuropathic Pain after Chemotherapy
References：Cell Reports

Tpl2 Protects Against Fulminant Hepatitis Through Mobilization of Myeloid-Derived Suppressor Cells
References：Frontiers in Immunology

Gut Microbial Metabolite Pravastatin Attenuates Intestinal Ischemia/Reperfusion Injury Through Promoting IL-13 Release From Type II Innate Lymphoid Cells via IL-33/ST2 Signaling
References：Frontiers in Immunology

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Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

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