hAPOE4

모델명

C57BL/6Smoc-Apoetm2(hAPOE4)Smoc

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NM-HU-190002

모델 상태

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유전자 요약

Official Symbol
Apoe

Synonyms

Apo-E; AI255918

NCBI ID

11816

MGI ID

88057

Ensembl ID

ENSMUSG00000002985

Pubmed

Apoe

Human Ortholog

APOE

모델 설명

This APOE4 humanized mouse model was established by replacing the mouse Apoe gene coding exons and introns with the human APOE4 corresponding sequence by homologous recombination.
신청분야：Cardiovascular disease

검증 데이터

Apoe4-2.png

Fig1. Genotyping of WT 、KO &  Humanized mice by PCR.

Primer P1/2 Wild type = 342 bp

Primer P3/4 mutant = 332 bp

M：100bp Plus DNA ladder.image.png

Fig2. Western blot analysis of APOE4 protein expression of WT C57, Apoe-KO and APOE4 humanized mice. 

image.png

Fig3. Detection of human APOE4 expression in brian in hAPOE4 knockin mice by IHC. 

Brian were collected from wild-type mice and HO hAPOE4 mice, and analyzed by IHC with anti-human APOE4 antibody. 

Abbr. HO, homozy gous; WT, wild type.

image.png

Fig4. Detection of human APOE4 expression in Cerebellum in hAPOE4 knockin mice by IHC. 

Brian were collected from wild-type mice and HO hAPOE4 mice, and analyzed by IHC with anti-human APOE4 antibody. 

Abbr. HO, homozygous; WT, wild type.

image.png

Fig5. Detection of human APOE4 expression in Cornu ammonis 1(CA1) in hAPOE4 knockin mice by IHC. 

Brian were collected from wild-type mice and HO hAPOE4 mice, and analyzed by IHC with anti-human APOE4 antibody. 

Abbr. HO, homozygous; WT, wild type.

image.png

Fig6. Detection of human APOE4 expression in Cornu ammonis 3(CA3) in hAPOE4 knockin mice by IHC. 

Brian were collected from wild-type mice and HO hAPOE4 mice, and analyzed by IHC with anti-human APOE4 antibody. 

Abbr. HO, homozygous; WT, wild type.

image.png

Fig7. Detection of human APOE4 expression in Dentate Gyrus(DG) in hAPOE4 knockin mice by IHC. 

Brian were collected from wild-type mice and HO hAPOE4 mice, and analyzed by IHC with anti-human APOE4 antibody. 

Abbr. HO, homozygous; WT, wild type.

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Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

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Milestones

Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.

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