hCRBN
모델명
C57BL/6Smoc-Crbntm1(hCRBN)Smoc
카탈로그
NM-HU-225071
모델 상태
Repository Live
유전자 요약
Official Symbol
Crbn
모델 설명
검증 데이터
Fig1. Detection of CRBN expression in spleen, brain, liver, intestine and muscle tissues by RT-PCR.
Wild type: only one band at 185 bp with primers F1/R1 (mCrbn);
Homozygous: one band at 262 bp with primers F2/R2 (hCRBN) and one band at 374 bp with primers F3/R3 (hCRBN);
Abbr. M, DNA marker; HO, homozygous; WT, wild type.
Fig2. Expression characterization of hFCRN in homozygous hCRBN KI mice by Western blot.
Fig3. The degradation of GSPT1, CK1a and Myc by Lenalidomide and CC-885 in hCRBN knockin mice were determined by western blot.
The splenocytes derived from wild-type C57BL/6 (WT) and homozygous hCRBN KI (HO) mice were treated with 1% DMSO, Lenalidomide (10 nM, 100 nM, 1000 nM) and CC-885 (10 nM, 100 nM, 1000 nM) ,respectively, in RPMI-1640 cell culture medium for 18 hours ex vivo.
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On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
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