Cx3cr1-Cre

모델명

C57BL/6Smoc-Cx3cr1em1(iCre)Smoc

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NM-KI-200079

모델 상태

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유전자 요약

Official Symbol
Cx3cr1

Synonyms

-

NCBI ID

13051

MGI ID

1333815

Ensembl ID

ENSMUSG00000052336

Pubmed

Cx3cr1

Human Ortholog

CX3CR1

모델 설명

The coding region of Cx3cr1 gene was replaced by a iCre expression cassette via CRISPR/Cas9 mediated recombination.
신청분야：Cre recombinase tool; Cx3cr1 exclusively expressed in the mononuclear phagocyte system including microglia, as well as hepatic kupffer cells and alveolar macrophages. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cells expressing cre. This strain may be useful for studying lung and liver fibrosis.

검증 데이터

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Fig. 1 Cre-mediated recombination in the liver of Cx3cr1Cre/+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the pulmonary macrophage of Cx3cr1Cre/+; Rosa26tdTomato/+ mouse.

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Fig. 2 Cre-mediated recombination in the liver of Cx3cr1Cre/+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the hepatic kupffer cells  of Cx3cr1Cre/+; Rosa26tdTomato/+ mouse.

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Fig. 3 Cre-mediated recombination in the brain of Cx3cr1Cre/+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the microglial cells of Cx3cr1Cre/+; Rosa26tdTomato/+ mouse.

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Fig. 4 Detection of tdTomato(red) in various tissues of Cx3cr1Cre/+; Rosa26tdTomato/+ mice. Cre mediated recombination can be detected in some cells of the brain, liver, lung, stomach, salivary gland, small intestine, spinal cord, spleen, testis and epididymis, skin and subcutaneous tissue, skeletal muscle and thymus. Tdtomato expression can not be observed in the brown fat, heart, retina, kidney or pancreas. (For more detailed information please contact our technical advisor.)


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Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

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Milestones

Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.

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