Cd8a-IRES-Luc-2A-EGFP

모델명

C57BL/6Smoc-Cd8aem1(V5-IRES-luci-2A-EGFP)Smoc

카탈로그

NM-KI-18030

모델 상태

Embryo cryopreservation

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유전자 요약

Official Symbol
Cd8a

Synonyms

Ly-2; Ly-B; Ly-35; Lyt-2; BB154331

NCBI ID

12525

MGI ID

88346

Ensembl ID

ENSMUSG00000053977

Pubmed

Cd8a

Human Ortholog

CD8A

모델 설명

A V5-IRES-luci-2A-EGFP expression cassette was inserted before the stop codon TAA of mouse Cd8a gene to establish a multi-labeled knockin model with V5, luciferase and EGFP fluorescent protein.
신청분야：gene tracing

검증 데이터

Cd8a-V5-IRES-Akaluc-2A-EGFP mouse model_05.png

Figure 1  In the absence of any stimulation, the in vivo imaging system detected that Cd8aV5-IRES-luci-2A-EGFP/+ mice showed significant fluorescence.

Cd8a-V5-IRES-Akaluc-2A-EGFP mouse model_06.png

Figure 2  In vivo imaging validation of MC38 tumors in Cd8aV5-IRES-luci-2A-EGFP/+ reporter mice treated or not with a PD-1 mAb. After 1 day of stimulation with PD1 antibody, the fluorescence was significantly enhanced.

Cd8aV5-IRES-luci-2A-EGFP-3.png

Figure 3  EGFP expression analysis in  PBMC of Cd8aV5-IRES-luci-2A-EGFP/+ mice by flow cytometry.

당신은 또한 좋아할 수 있습니다

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

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Milestones

Our vision is to provide researchers all over the world with comprehensive, convenient and professional animal model services to facilitate a simplified and highly-efficient approach towards uncovering the mysteries of life.

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